Re: [Histonet] DAB residues removal

From:Rene J Buesa

If it is just to clean glassware, place it in bleach until the stain is removed.
  If that glassware is to be used again for IHC procedures, you have to be ABSOLUTELY sure that ALL the bleach resideues have been eliminated.
  René J.

Olek Michalski  wrote:
Dear Histonetters,

There are many procedures using DAB for staining. We apply some in our 
lab, so sometimes there is need for cleaning Coplin jars covered w/ brown 
residue. I normally do it by putting glassware into NaOH solution 
containing some detergent over weekend and cleaing w/ brush afterwards. 
Recently I heard there used to be more efficient protocol for DAB residue 
removal but I can't find it. I know Dako provides DAB-away kit, but 
supposedly it would be too much for couple jars per week. Do you know any 
better way to deal with this problem?

Best regards
Olek Michalski

Laboratory of Neurobiology
of Development and Evolution

Nencki Institute of Experimental Biology
ul. Pasteura 3, 02-093 Warszawa, Poland
Tel. +48 22 5892268, Fax +48 22 8225342

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