[Histonet] DAB residues removal

From:"Olek Michalski"

Dear Histonetters,

There are many procedures using DAB for staining. We apply some in our  
lab, so sometimes there is need for cleaning Coplin jars covered w/ brown  
residue. I normally do it by putting glassware into NaOH solution  
containing some detergent over weekend and cleaing w/ brush afterwards.  
Recently I heard there used to be more efficient protocol for DAB residue  
removal but I can't find it. I know Dako provides DAB-away kit, but  
supposedly it would be too much for couple jars per week. Do you know any  
better way to deal with this problem?

Best regards
Olek Michalski

         Laboratory of Neurobiology
        of Development and Evolution

  Nencki Institute of Experimental Biology
  ul. Pasteura 3, 02-093 Warszawa,  Poland
  Tel. +48 22 5892268,  Fax +48 22 8225342

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