[Histonet] The use of reducing agents in antigen retrieval?
We have been trying (unsuccessfully) to use a polyclonal antibody raised against a peptide in some IHC runs. The antibody recognizes antigen by western blotting only under reducing conditions (i.e. DTT or 2-mercaptoethanol). Thus, it appears that the peptide/epitope is masked by disulphide bond(s). Is there an antigen retrieval method out there that incorporates reducing conditions? Any help will be appreciated.
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