Why do you want to thaw it if you are putting it in OCT and cryosectioning??
Sharron

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee
Fish
Sent: Thursday, June 10, 2004 2:22 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Embedding previously frozen brain


Hello Netters,
I have a question that came from an investigator at my institute:

Can mouse brain that has been snap frozen in liquid nitrogen (stored 
at -80 C degrees) be thawed and embedded in OCT for cryosectioning 
without causing excessive damage?  How can this be done?

I appreciate all of your answers and thank you all in advance.

Take care,
Jo Dee


********************************************************************** 
**********

Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 695-3720
Fax: (415) 285-5632
E-mail: jfish@gladstone.ucsf.edu

Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100

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