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From: | Nancy.Walker@sanofi-synthelabo.com |
Axons stain nicely with beta 3 tubuline (TUJ). We've used the following
antibodies
Monoclonal anti-beta-tubulin isotype III from Sigma 1/1000 DAKO envision
(révélation PAL or HRP) (ref : T8660)
or
Polyclonal (rabbit) antibody against neuronal class III beta-tubulin from
covance research commercialise par BABCO Berkeley antibody company au
1/1000 DAKO envision (révélation PAL or HRP) (ref : PRB-435P)
We used paraffin slices of PFA fixed rat embryos and adult rat brain,
microwaving in citrate buffer. These antibodies also work nicely with IF
Good luck!
Nancy Walker
Molecular Biology Scientist
Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE
nancy.walker@sanofi-synthelabo.com
tel : (33)561004179 fax :(33)561004001
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