re fixation for frozen sections

From:Carl Hobbs

".....It is not melted on a room temperature slide in
the mounting step which causes the quality to degrade".
This is a from comment made referring to the std practice for mounting
cryosections to slides( non- Jane). Actually, my understanding was that the
section is not, in fact melted on... the temp differential between RT slide
and section temp and the time taken to mount it, is sufficient for the
section to dry onto the slide by sublimation? Hence the white powder left on
the knife.
However, I would be interested to see side-by-side pics of Jane and Std
Carl Hobbs
Laboratory Manager
Molecular Neurobiology Group
MRC Centre For Developmental Neurobiology
New Hunts House
Guys Campus
kings College London
020 7848 6810/fax 6816

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