Deparafinize sections as usual, stain with 1% Uvitex 2B in PBS for 10-30 
min RT, rinse with saline, counterstain with 0,5% Evans Blue in PBS for 
30s, or with Gill's hematoxylin. Dehydrate, clear in xylene and mount.

Sometimes background staining is caused by autofluorescent mounting 
media and/or counterstains. Use these counterstains and avoid 
autofluorescent resisns for mounting. Hope it helps,

Oswaldo


Louro, Pedro wrote:

>I'm looking for any immunohistochemistry protocol used to stain
>Microsporidia on paraffin blocks.
>Our group is on a stand still with lots of background staining.
>
>Any help would be greatly appreciated.
>
>Thanks
>
>
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