RE: Oil Red O

From:Tony Henwood

RE: Oil Red O


I would rinse the tissue in water (to remove excess formalin), impregnate with 30% OCT in water 3-4 hours (or a sucrose substitute), blot to remove excess solution and freeze ready for cryotomy.

Use sticky slides (Superfrost or equivalent) to mount sections.

The Churukian Oil Red O procedure works well.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Sean Trombley []
Sent: Thursday, 12 June 2003 5:13
To: histonet
Subject: Oil Red O

Hi Everyone!

I was hoping to get a protocol for Oil Red O  on NBF fixed frozen mouse liver
and intestine.  I would appreciate a freezing protocol as well.   We are having
a debate in our lab as to the best way to approach this.  Thanks in advance for
the help!

Bayer Corp
W. Haven, CT

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer

<< Previous Message | Next Message >>