| From: | Tony Henwood |
Sean,
I would rinse the tissue in water (to remove excess formalin), impregnate with 30% OCT in water 3-4 hours (or a sucrose substitute), blot to remove excess solution and freeze ready for cryotomy.
Use sticky slides (Superfrost or equivalent) to mount sections.
The Churukian Oil Red O procedure works well.
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----
From: Sean Trombley [mailto:sean.trombley.b@bayer.com]
Sent: Thursday, 12 June 2003 5:13
To: histonet
Subject: Oil Red O
Hi Everyone!
I was hoping to get a protocol for Oil Red O on NBF fixed frozen mouse liver
and intestine. I would appreciate a freezing protocol as well. We are having
a debate in our lab as to the best way to approach this. Thanks in advance for
the help!
Sean
Bayer Corp
W. Haven, CT
(203)812-5471
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