More questions on DNA staining

From:"Johnson, Teri"

What fixative is recommended for the Feulgen stain in
paraffin-embedded tissue? Carson recommends any fixative
except Bouins, Humason recommends a fixative containing
Mercuric chloride (but offers a splendid chart for
hydrolysis times according to alternate fixatives),
and Kiernan recommends avoiding picric acid and
any acid decalcifiers.  Because I'm not tied to 
routinely fixed (10% NBF) tissues, I can use
whatever is optimal. 

Which is...?

I suspect the same fixative agent should be used
regardless of technique (fluorescence vs.feulgen).

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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