More questions on DNA staining
What fixative is recommended for the Feulgen stain in
paraffin-embedded tissue? Carson recommends any fixative
except Bouins, Humason recommends a fixative containing
Mercuric chloride (but offers a splendid chart for
hydrolysis times according to alternate fixatives),
and Kiernan recommends avoiding picric acid and
any acid decalcifiers. Because I'm not tied to
routinely fixed (10% NBF) tissues, I can use
whatever is optimal.
I suspect the same fixative agent should be used
regardless of technique (fluorescence vs.feulgen).
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
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