LN2 snap freezing vs -80?
Has anyone had any experience with LN2 snap freezing vs. slow freezing at
-80. I was wondering if there is a difference in ease of cryosectioning, RNA
stability, morphology, etc... The samples are all embedded in OCT first.
Does the formation of ice crystals by slow freezing cause tissue to tear
when crysectioning?
thanks.
jason.
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