osmium replies

From:Anne Undersander

Thanks everyone for the osmium replies.  We did suggest using two different pieces of tissue for the two different studies, however, our opinion was asked only after the fact. 
The tissue was fixed in glutaraldehyde then postfixed in osmium.  The EM procedures were done, then the tissue was washed and placed in 70% ETOH.  Now, we have it and are suppose to decal, process, cut and H&E stain it.  It sounds like the quality of these sections will be poor, if at all.  Gayle- we'll see if the EDTA will work. 
Thanks again!  Please let me know if there's anything else to consider.
Anne Undersander HT(ASCP)
Vet. Med./Animal Science
University of Minnesota

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