If this is the case then I would just use one of the many antigen 
retrieval methods developed for IHC.

Bob
Robert Schooonhoven

Tague, Curtis wrote:

>I read somewhere that if left in formalin too long there will be a problem with staining. Something to do with a cross link blocking of a protein on the membrane. Anyone familiar with the details of this and if this is accurate, is there a way to reverse the action, soak in 70% or something like that? Or, is that tissue just lost?
>
>We have a few lobes of lung that are/were all loaded but don't stain now.
>
>Thanks,
>Curt
>
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