cold decalc

From:Joey Shepherd

Hi everyone
Does anyone else out there use EDTA decalcifcation at 4'C? Its been 
reccomended to me for good IHC results. If so what strength/recipe do 
you use and how long does it take? I'm currently using a 20% EDTA 
solution at room temperature on mouse hind limbs - it seems to take 
forever and my antigen retrieval for IHC is pretty bad (although I 
strongly suspect that's due to very long periods spent floating in 
formalin - many of these are archived samples!).


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