Re: MYELIN STAIN

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IHC World

MYELIN STAIN

I always use gelatin coated slides to mount brain tissue sections. I have been cutting 50 um paraffin sections and mounted on getatin coated slides stained with LFB for myelin and worked very well. However, sections falling off slides may have something to do with fixation as well.

Richard
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----- Original Message -----

From: Maliniak, Richard

To: histonet@pathology.swmed.edu

Sent: Wednesday, July 02, 2003 1:38 PM

Subject: MYELIN STAIN

We have not been able to do a myelin (special stain or immunostain) on an autopsy specimen because the brain section keep falling off the slides. We have tried all types of slides including charged slides and no work. Any suggestions on what we should do? Would anyone like us to send them the slides to try?

Rick

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