RE: re HID/AB pH 2.5

From:Tony Henwood

RE: re HID/AB pH 2.5

Have any of you tried the Shikata's Orcein/AB stain instead. Definitely easier and SAFER to do.

Singh, R., Gorton, A.W.P., (1989) "Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium" J Clin Pathol 42:881-4.

Henwood, A., (2002) "The Orcein Stain- A versatile stain for histopathology" J Histotechnol 25:29-31.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Gutierrez, Juan []
Sent: Thursday, 3 July 2003 3:03
To: 'Kim Saunders';
Subject: RE: re HID/AB pH 2.5

Cursed be all those pathologists!  I'm having the same problem with mine.
So far I haven't had any luck getting the right kind of staining(my nuclei
turn green).  If anyone could pleaaaaasssseeee forward me a copy of a good,
proven, often immitated but never duplicated, the kind of procedure to take
home to meet mother,  I will be forever grateful.
Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Hospital

-----Original Message-----
From: Kim Saunders []
Sent: Tuesday, July 01, 2003 8:19 PM
Subject: re HID/AB pH 2.5

Is anyone familiar with this special stain "High Iron Diamine/alcian blue pH
2.5" ? If so, can you share your protocol with me. My pathologists are very
interested in this one.
Kim Saunders, HT
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Kim Saunders
Proud to be an American!!


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