PCR and microtomy
Message
We have been asked
to cut 20 micron sections from paraffin blocks for PCR. My question is how
do others clean/decontaminate the microtome, blades, etc that are used. Do
you also sterilize any forceps or other instruments used, and does the tech wear
sterile gloves? Since the tissue was not treated specially up to this
point (we did not know of the pcr request) does it really matter that everything
is sterile now? We did purchase DNAase and RNAase free vials to put the
ribbons in.
Audrey Pagan
Laboratory
Manager
Lakewood Pathology
Associates
1200 River Ave. Suite 10E
Lakewood, NJ 08701
732-901-7575 ext. 102
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