validation of antibodies

From:"Corazon D. Bucana"

Recently some questions came up on how best to validate the antibodies that
we are using.  I would appreciate any information as to how other people do
this sort of thing.  My strategy is to do a competition experiment but
sometimes that may not be feasible especially when dealing with antibodies
to phosphorylated molecules.  Any comments or suggestions would be greatly

Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

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