Whole mouse snap freezing for frozen sections

From:Gayle Callis

I was asked how people snap freeze a euthanized whole mouse for serial
frozen sections.  I've snap frozen whole mouse heads, tibias, femurs using
dry ice cooled hexanes after coating the samples with either diluted OCT or
4% PVA, 30,000 - 70,000 water soluble from Sigma. Is this snap freezing
method going to be too slow for a whole mouse. Any help would be
appreciated.  Off to PubMed.

Searching the Archives was not turning up the answer

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (voice mail)
406 994-4303 (FAX)

email: UVSGC@montana.edu

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