> I am curious in general if anyone has had success staining tissue sections for beta-gal. We have been able to get it to work in cultured fibroblasts, but when it comes to tissue sections we have had no luck. Just curious to if any of you have had success and if so if I could get your protocol. Thanks in advance.

Kara 
> From: r-meyer2@northwestern.edu
> Date: 2002/06/24 Mon PM 04:15:33 EDT
> To: histonet@pathology.swmed.edu
> Subject: x-gal staining fixation
> 
> 
> 
> We have just started doing x-gal staining in our lab on frozen sections.  A 
> researcher brought us b-gal positive control liver tissue to use to work out 
> the stain.  Several of the protocols we looked up including those found in 
> histonet archives use a prefixation step before freezing the tissue in liquid 
> nitrogen.  We found out that the researcher did not do any prefixation and only 
> froze the tissue before giving it to us.  Does anyone know how important this 
> step is, and is this possibly the reason why we are not getting any staining??? 
> Thanks for any input! 
> 
> Bob Meyer 
> Pathology Core Facility 
> 
> 
> 
> 
>