Re: animal tissue
I, afterworking in research at at Cornell Univ veterinary college have
found no difference in processing animal tissue with the exception of the
small sections, but I believe you would encounter the same brittle results
if you were dealing with any small tissue. I agree with you Laurie, human
uterus is the pits to work with. Amy, just process as usual and you should
have no problems.
----- Original Message -----
From: "Laurie Reilly"
To: "Monson, Frederick C." ;
Sent: Thursday, June 27, 2002 8:50 PM
Subject: RE: animal tissue
> G'day Amy and Fred,
> My experience with animal tissues concurs with what Fred has said; humans
> are very similar to other animals, histologically speaking. Having trained
> in a hospital and then moved into the veterinary field I found little
> difficulty transposing methods from one to the other.
> Certainly, most bovine tissues can be treated just the same as human
> tissues. One difference being that bovine uterus is a "piece of cake" to
> cut compared to my memories of human uterus.
> The other consideration with animal tissues is that small specimens,
> particularly from rodents and small birds, require much shorter processing
> times if they are not to become too brittle to cut.
> Regards, Laurie.
> At 04:44 PM 06/27/02 -0400, Monson, Frederick C. wrote:
> >Hi Amy,
> > In my experience, whether processing amphibine, reptiline, or
> >mammaline (including primatine (including humine), lagormorphine,
> >porcine, elephantine or whaline) tissues, the procedures only varied as a
> >consequence of known pathology (sp., pathogines). As you can see I have
> >steer'd clear of bovines, because I never permitted myself to be cowed
> >working with such bull. Actually, however, someone snuck (sneaked?) some
> >bovide tissue into a run years ago and I didn't know the difference until
> >after I had included the results in my rodentide report.
> > My advice is to steer clear of any procedural modification that
> >the bovid apart from the same processing used on other mammalids
> >Best regards and good luck on your boars. I'll soon be taking the
> >in Advanced English with a concentration in phonetic consistency as soon
> >I save sufficient funds.
> >Fred Monson - someone tell me it's not close to Friday!
> >Frederick C. Monson, PhD
> >Center for Advanced Scientific Imaging
> >Schmucker II Science Center
> >West Chester University
> >South Church Street and Rosedale
> >West Chester, Pennsylvania, USA, 19383
> >Phone: 610-738-0437
> >FAX: 610-738-0437
> >CASI URL: http://darwin.wcupa.edu/casi/
> >WCUPA URL: http://www.wcupa.edu/
> >Visitors URL: http://www.wcupa.edu/_visitors/
> > > ----------
> > > From: Amy30histo@cs.com
> > > Sent: Thursday, June 27, 2002 3:46 PM
> > > To: firstname.lastname@example.org
> > > Subject: animal tissue
> > >
> > >
> > > Does anyone have any info. or protocols/procedures that they
> > > be willing to share on processing and staining of animal tissue? I
> > > with human tissue but am gonna have to use some animal tissue(steer)
> > > my histology boards - Any info. would be appreciated -
> > >
> > > Amy
> > > 843-527-7179 - phone
> > >
> > > 843-520-7882 - fax
> > >
> Mr.Laurie Reilly Ph 07 4781
> Physiology & Pharmacology Fax 07 4779 1526
> Aust.Inst.of Tropical Vet.& Animal Sc.
> James Cook University
> Townsville Qld.
> 4811 email@example.com
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