Re: CD31 in FFPE mouse tissues
I just completed a project usind CD31 on FFPE mouse tissue with good results.
The supplier of the antibody is Research Diagnostics (973-584-7093;
www.researchd.com). I used rat anti-mouse CD31, monoclonal; Catalog#
RDI-mCD31abrt. They also suggested zinc fixation, but it worked on formalin
fixation. I used DAKO's Prot K to open up the sights.
Pathology Core Facility
> I'm looking for a supplier of CD31 to stain formalin-fixed, paraffin embedded
mouse tissue. I understand some companies offer antibodies that don't work
well in formalin fixed material, but instead suggest using zinc fixed tissue
(not zinc formalin fixed). Unfortunately, I don't happen to have any zinc
fixed tissue at the moment.
> Thanks in advance!
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri 64110
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