Re: CD31 in FFPE mouse tissues
From: | r-meyer2@northwestern.edu |
Teri,
I just completed a project usind CD31 on FFPE mouse tissue with good results.
The supplier of the antibody is Research Diagnostics (973-584-7093;
www.researchd.com). I used rat anti-mouse CD31, monoclonal; Catalog#
RDI-mCD31abrt. They also suggested zinc fixation, but it worked on formalin
fixation. I used DAKO's Prot K to open up the sights.
Bob Meyer
Pathology Core Facility
Northwestern University
>
> I'm looking for a supplier of CD31 to stain formalin-fixed, paraffin embedded
mouse tissue. I understand some companies offer antibodies that don't work
well in formalin fixed material, but instead suggest using zinc fixed tissue
(not zinc formalin fixed). Unfortunately, I don't happen to have any zinc
fixed tissue at the moment.
>
> Thanks in advance!
>
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri 64110
> tjj@stowers-institute.org
>
>
<< Previous Message | Next Message >>