Re: CD31 in FFPE mouse tissues


I just completed a project usind CD31 on FFPE mouse tissue with good results.  
The supplier of the antibody is Research Diagnostics (973-584-7093;  I used rat anti-mouse CD31, monoclonal; Catalog# 
RDI-mCD31abrt.  They also suggested zinc fixation, but it worked on formalin 
fixation.  I used DAKO's Prot K to open up the sights. 

Bob Meyer 
Pathology Core Facility 
Northwestern University 

> I'm looking for a supplier of CD31 to stain formalin-fixed, paraffin embedded 
mouse tissue.  I understand some companies offer antibodies that don't work 
well in formalin fixed material, but instead suggest using zinc fixed tissue 
(not zinc formalin fixed).  Unfortunately, I don't happen to have any zinc 
fixed tissue at the moment. 
> Thanks in advance! 
> Teri Johnson 
> Managing Director Histology Core Facility 
> Stowers Institute for Medical Research 
> 1000 E. 50th St. 
> Kansas City, Missouri  64110 

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