Re: FW:

From:Bryan Hewlett


Suspend bacterial pellet in 200microliters of plasma, 
add 150 microliters of thrombin, 
wait 15 minutes for clot to form and retract, 
fix in NBF and process to wax as a tissue block


Bryan R. Hewlett
Technical Specialist
Anatomical Pathology
Hamilton Regional Laboratory Medicine Program
Ontario, Canada

----- Original Message ----- 
From: "Cindy Chard-Bergstrom" 
Sent: Wednesday, June 19, 2002 12:10 PM
Subject: Re: FW:

> Dear Histonetters,
> Does anyone have a method of processing and embedded a small pellet of
> bacteria. I am working on a research project and I need the bacteria for
> a positive control. Thanks to all in advance.
> Cindy Chard-Bergstrom B.S.,HT(ASCP),QIHC
> Microbiologist III 
> Kansas State University

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