Cindy,

Easy! 
Suspend bacterial pellet in 200microliters of plasma, 
add 150 microliters of thrombin, 
wait 15 minutes for clot to form and retract, 
fix in NBF and process to wax as a tissue block

Bryan

Bryan R. Hewlett
Technical Specialist
Anatomical Pathology
Hamilton Regional Laboratory Medicine Program
Ontario, Canada

----- Original Message ----- 
From: "Cindy Chard-Bergstrom" 
To: 
Sent: Wednesday, June 19, 2002 12:10 PM
Subject: Re: FW:


> Dear Histonetters,
> Does anyone have a method of processing and embedded a small pellet of
> bacteria. I am working on a research project and I need the bacteria for
> a positive control. Thanks to all in advance.
> 
> 
> 
> Cindy Chard-Bergstrom B.S.,HT(ASCP),QIHC
> Microbiologist III 
> Kansas State University
> chardb@vet.ksu.edu
> 
>