correction, the DAKO autostainer applies DI water not buffer after the
run is done to keep the slides wet.
patsy

Noreen Gilman wrote:

>  Christina, I do run the autostainer overnight, but I don't use the
> delay. I find that I use much more buffer than if I just ran the
> program and left it alone until the next morning. The machine keeps
> the slides wet with distilled water and I don't waste the expensive
> buffer. I've not had any problems doing this.Noreen Noreen Gilman,
> B.S., H.T.(ASCP) CLS
> Histopathology Supervisor
> Broward General Medical Center
> Ft. Lauderdale, FL 33316
> 954.355.5592 Phone
> 954.355.4139 Fax
> 954-387-0213 Pager
>
> >>> C Sawrenko  06/11/02 09:30AM >>>
> Hello Histonetters,
> Thanks for your timely response to our ISH pretreatment question.  I
> now
> have another one for Dako autostainer users.  Do any of you run your
> autostainers overnight using the delay option?  How often?  Any
> problems
> encountered?  Any thrifty souls reusing the reagent vials?
> Thanks in advance for your valuable input!
>
> Christina Sawrenko
> Histopathology
> BC Cancer Agency
> Vancouver, BC
>
>
>