I also use DAKO's Prolactin at 1:800, but with microwave pretreatment for 2
x 5 minutes in citrate buffer.  I found that I get more intense positive
staining and less nonspecific background with the MW pretreatment.

Jan Shivers
U of MN

----- Original Message -----
From: "Greg Dobbin" <dobbin@Upei.CA>
To: <Histonet@pathology.swmed.edu>
Sent: Friday, June 22, 2001 11:34 AM
Subject: IHC-Prolactin & Chromophore


> Hello Friends,
>
> Can someone help me out with a few pointers to get me started on
> IHC for prolactin and pituitary chromophore in FFPE tissues,
> (i.e. Ab source and Ag retreival method, if needed)?
> Thanks in advance!
> Greg
>
>
>
>