|From:||"Dawson, Glen" <GDawson@Milw.Dynacare.com>|
Before going to another antibody, try adding IgM to your Link step (About a
1:100 ratio of IgM to Link). This helped my CD15 immensely. Link is a DAKO
detection term so for ventanna it may be called the biotinylated secondary
step? I add about 100ul to around 10ml of link.
Glen Dawson BS, HT & IHC (ASCP)
Lead IHC Technologist
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG]
Sent: Wednesday, June 27, 2001 3:44 PM
Just wondering what other labs are using for a CD15 antibody to detect Reed
Sternberg cells. Our CD15 has become very inadequate on our NexES - even
with a heat retrieval. Currently we are using Ventana's product (clone
Thanks in advance.
Fairview-University Medical Center
Mpls. MN 55454
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