RE: CD15

From:"Dawson, Glen" <>


Before going to another antibody, try adding IgM to your Link step (About a
1:100 ratio of IgM to Link).  This helped my CD15 immensely. Link is a DAKO
detection term so for ventanna it may be called the biotinylated secondary
step?  I add about 100ul to around 10ml of link.

Good Luck,

Glen Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG]
Sent: Wednesday, June 27, 2001 3:44 PM
To: histonet@pathology.swmed.EDU
Subject: CD15

Hi Histonetters,

Just wondering what other labs are using for a CD15 antibody to detect Reed
Sternberg cells.  Our CD15 has become very inadequate on our NexES - even
with a heat retrieval.  Currently we are using Ventana's product (clone

Thanks in advance.

Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454

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