RE: CD15
From: | "Dawson, Glen" <GDawson@Milw.Dynacare.com> |
Ann,
Before going to another antibody, try adding IgM to your Link step (About a
1:100 ratio of IgM to Link). This helped my CD15 immensely. Link is a DAKO
detection term so for ventanna it may be called the biotinylated secondary
step? I add about 100ul to around 10ml of link.
Good Luck,
Glen Dawson BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG]
Sent: Wednesday, June 27, 2001 3:44 PM
To: histonet@pathology.swmed.EDU
Subject: CD15
Hi Histonetters,
Just wondering what other labs are using for a CD15 antibody to detect Reed
Sternberg cells. Our CD15 has become very inadequate on our NexES - even
with a heat retrieval. Currently we are using Ventana's product (clone
MMA).
Thanks in advance.
Ann Maruska
Fairview-University Medical Center
Mpls. MN 55454
amarusk1@fairview.org
612-273-9119
<< Previous Message | Next Message >>