Re: Mouse anti E--Cadherin
|From:||Richard Cartun <Rcartun@harthosp.org>|
We use Zymed's E-Cadherin mAb at 1:200 for 30' (1:1,000 for overnight incubation) with DAKO's EnVision+ detection. A 40' incubation in a 96 degree C. waterbath is used for HIER. Try a ductal carcinoma of breast for your positive control.
>>> THERESA ROHR <firstname.lastname@example.org> 06/12/01 04:31PM >>>
I just received my order of E-Cadherin from Zymed. If anyone uses their
concentrate with the Dako Envision Plus protocol, please let me know what
dilution you are currently using. Also, are you using HIER and protein
blocking as recommended and the 60 minute incubation time? What works really
well as a control tissue??
Theresa Rohr, BA HT(ASCP)
Section Head, Histology
Nyack Hospital, NY
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