RE: cytokine staining cultured cells

From:Gayle Callis <>

And if you want to see them as colored endpoints, with a chromogen ie DAB,
you can detect with an antiFITC conjugated to HRP.  Intracellular cytokine
stains can require approx 10ug/ml to detect, and having concentrated
antibodies gives one a bit more leeway for the correct concentration for
detection via light microscope.   

Question:  are these designed for FACS analysis applications?? 

At 04:53 PM 6/13/01 -0600, you wrote:
>eBioscience also carries pretitrated, fluorochrome-conjugated cytokine
>antibodies for intracellular staining.
>For product listing and protocol, please visit:
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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