"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
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<blockquote type=cite class=cite cite>> Good Morning,<br>
> Is there anyone out there that is still hand staining large volumes
of<br>
> Immunos? I am trying to locate the plastic humidity chambers that
hold<br>
about<br>
> 10 slides each. If anyone has a contact could you please let me
know? Also<br>
> if anyone has a better idea let me know, I just got a request for
250<br>
BrDU's<br>
> :)<br>
> Mary</blockquote><br>
Mary,<br>
<x-tab> </x-tab>That's me,
immunostainers are for softies. All you need is a friendly mechanical
workshop.<br>
Humidity chamber. Go to your EM Unit or photographer and ask for some old
glass plates. Take as many as they will give they're really handy. Put
them into hot water and strip off the gelatine, wash and dry. Take the
plates to the workshop and ask if they will make a box using the glass
plates as sliding shelves about 1cm apart. My boxes have six such shelves
but the number you choose will be up to yourself. A glass EM plate holds
3 slides so my box takes 18 slides and I can assure you 2 boxes is as
much as you want in an IHC run doing it by hand. As for the humidity
chamber itself, easy, I use a clear plastic lunch box. One size takes 1
slide box and another I use takes 2. Dampen an few sheets of Kleenex, pop
into lunch box and that will supply the humidity. The lunch boxes can
then go into the fridge or an orbital shaker.<br>
Ian. <br><br>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>