Fwd: Fw: humidity chambers

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>

<html> <blockquote type=cite class=cite cite>> Good Morning,<br> > Is there anyone out there that is still hand staining large volumes of<br> > Immunos? I am trying to locate the plastic humidity chambers that hold<br> about<br> > 10 slides each. If anyone has a contact could you please let me know? Also<br> > if anyone has a better idea let me know, I just got a request for 250<br> BrDU's<br> > :)<br> > Mary</blockquote><br> Mary,<br> <x-tab>        </x-tab>That's me, immunostainers are for softies. All you need is a friendly mechanical workshop.<br> Humidity chamber. Go to your EM Unit or photographer and ask for some old glass plates. Take as many as they will give they're really handy. Put them into hot water and strip off the gelatine, wash and dry. Take the plates to the workshop and ask if they will make a box using the glass plates as sliding shelves about 1cm apart. My boxes have six such shelves but the number you choose will be up to yourself. A glass EM plate holds 3 slides so my box takes 18 slides and I can assure you 2 boxes is as much as you want in an IHC run doing it by hand. As for the humidity chamber itself, easy, I use a clear plastic lunch box. One size takes 1 slide box and another I use takes 2. Dampen an few sheets of Kleenex, pop into lunch box and that will supply the humidity. The lunch boxes can then go into the fridge or an orbital shaker.<br> Ian.   <br><br> <x-sigsep><p></x-sigsep> <font color="#0000FF">Dr. Ian Montgomery,<br> West Medical Building,<br> University of Glasgow,<br> Glasgow,<br> G12 8QQ.<br> Tel: 0141 339 8855.  Extn:6602.<br> Fax: 0141 330 2923<br> e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
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