Morphology of blood smears and peroxidase-rich tissues could be damaged by the aqueous hydrogen peroxide solutions. For such tissues, inactivate peroxidases by dipping the slides in 0.3% H2O2 in methanol for 10 min after during the dewaxing and rehydration step.
Abizar
www.innogenex.com

Bruce Abaloz wrote:

> Hi Histonetters, My name is Gratiana & I'm a PhD student @ The Uni. of Melbourne Australia. My question is what % of methanol that normally used to dilute Hydrogen peroxidase for Hydrogen peroxide quenching in immunohistochemistry.  Thankyou very much for your suggestion.
> Regard
> Gratiana
>
> BRUCE ABALOZ
> HISTOLOGIST
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