Re: % Of Methanol Used to Dilute....

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From:Greg Dobbin <dobbin@Upei.CA>
To:Bruce Abaloz <>,
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I don't intend this in a mean spirit, but what you mean to say is what 
dilution is used to dilute the hydrogen peroxide for the quenching of 
endogenous peroxidases. And, in short, 3% solution is commonly 
the concentration of choice. I have also seen 0.3% reccommended. 
Go  ahead and try 0.3%, and if you still have nonspecific staining 
(evident in your deletion control), then go to 3% the next time.

A cheaper alternative, if you are interested, is 3% in distilled water. 
I use it all the time and works great! Good luck! Cheers! Greg

Date sent:      	Fri, 23 Jun 2000 01:20:55 +1000
From:           	Bruce Abaloz <>
Subject:        	% Of Methanol Used to Dilute....
Forwarded to:

> Hi Histonetters, My name is Gratiana & I'm a PhD student @ The Uni. of Melbourne Australia. My question is what % of methanol that normally used to dilute Hydrogen peroxidase for Hydrogen peroxide quenching in immunohistochemistry.  Thankyou very much for your suggestion.
> Regard
> Gratiana
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851

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