----- Original Message -----

From: horalka@iopener.net
To: ryan2@niehs.nih.gov
Subject: soaking blocks
Date: Fri, 23 Jun 2000 15:15:37 -0600

We soak our blocks on ice blocks, keeping a small bottle of 25% ammonium hydroxide on the side to soak bloody tissues...we thought we were doing great until one day one of our pathologists had us recut some blocks because it appeared that the H&E stain was not optimal because they had soaked too long...I prefer using a dampened gauze on the block as I cut, instead of soaking them(too long).RS,RMC Denver
----- Original Message -----

From: ryan2@niehs.nih.gov
To: 'histonet' <histonet@pathology.swmed.edu>
Subject: soaking blocks
Date: Fri, 23 Jun 2000 11:25:32 -0400

How many out in HistoLand soak your blocks in ice water with ammonium 
hydroxide added?  What are the pros and cons(other that the odor!) of using 
ammonium hydroxide? 
 
Thanks   
 
Linda Ryan, BS, HT(ASCP)HTL 
National Institute of Environmental Health Sciences 
111  T. W. Alexander Dr. 
Bldg./rm.: 101/C-262 
P.O. Box 12233 
Research Triangle Park, NC 27709 
(919) 541-4880(Laboratory)