tissue section preparation

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From:Xiangyang Zhu <xzhu@UDel.Edu>
To:HistoNet Server <histonet@pathology.swmed.edu>
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Hi, everyone:
I am going to do the Fluorescent In Situ Hybridization (FISH) on cross-section of
intestinal tract. For the tissue preparation, I am considering using Cryostat or
embedding method. I am concerning the preservation of tissue structure, especially
the mucous layer. Does anyone know which method (Cryostat or embedding method)
work better to preserve the structure of mucous layer? Another question is the
thickness of the tissue section. What thickness is preferred for the Confocal
scanning. I am wondering if the very thick tissue section will stick to silane or
poly-lysine treated slides during the whole procedure of hybridization. Your
responses are appreciated.

Xiangyang Zhu
Research Associate
University of Delaware
Newark, DE 19717

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