RE: Her 2 by FISH

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From:Carlos Genty <cgenty@bcm.tmc.edu>
To:histonet@pathology.swmed.edu
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Hi Debbie:

Vysis, hands down.  They are all over the literature. The kit is FDA
approved and includes Cep 17 probe for proper signal enumeration.  If you
notice, most other probes give qualitative information, whereas Vysis' will
allow you to extract quantitative data.  The other probes are great for
difficult IHC cases (1+, 2+ positives) but will not give you anything
quantitative.

If cost is a factor, note that Vysis is very expensive and will require
flourescence and longer evaluation times per cell.

Also keep in mind that there is also a small percentage of cases that show
overexpression w/o amplification.

Good luck!

Carlos Genty
Breast Center Pathology
Baylor College of Medicine
Houston, Texas
cgenty@bcm.tmc.edu

-----Original Message-----
From: Jennings-Siena, Debbie [mailto:ds.jennings-siena@baylordallas.edu]
Sent: Tuesday, June 06, 2000 6:21 PM
To: 'histonet@pathology.swmed.edu'
Subject: Her 2 by FISH


If your lab is doing Her 2 by Fluorescent In-situ Hybridization techniques
(FISH) could you please tell me which manufacturer's kit that you are using
and how you like it?  My pathologist would like to know whose kit has the
highest reputation, I would also like to know about reputation but also ease
of use and reliability and costs.  Thanks for your help with this request.
Debbie J. Siena
Histopathology manager
Baylor University Medical Center
Dallas, Tx 75246
214-820-2465





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