Rosalba,

An Alcian blue pH1.0 or less, followed by a dilute 1:5000 Eosin should do
the job!

Bryan

> ----------
> From: 	rzumbo[SMTP:rzumbo@iofm.cs.nsw.gov.au]
> Sent: 	June 13, 2000 12:23 AM
> To: 	HISTONET@pathology.swmed.edu
> Subject: 	Eosinophils
> 
> Hi Histonetters,
> I am having a bit of trouble locating a dye to do a stain for 
> Eosinophils. The stain is Kass-Cooper-Choi method for Eosinophils 
> from Luna. It calls for a dye called Acid red 137 (possibly Alcian 
> Crocein), does anyone know if it has another name.
> Also does anyone know of a good stain for parrafin section to 
> distinguish eosinophils from Mast cells
> Ta 
> Rosalba Zumbo
> Institute of Forensic Medicine 
> Sydney
> Australia
>