yes, you can stain for those markers
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| From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
| To: | histonet@Pathology.swmed.edu |
| Reply-To: | |
| Date: | Fri, 16 Jul 1999 09:18:09 -0600 |
| Content-Type: | |
Yes, it is possible to stain for both of those markers, best done with
antimouse antibodies, and you may have better luck with frozens/acetone
fixed (PharMingen recommendation) on CD69. They indicated the thymus for
CD69 with frozens, the PNAd both frozens/paraffin with citrate retrieval,
and on the latter, using LN and small intestine (positive controls)
Our lab does PNAd aka Meca 79 on frozens with great success.
Good luck
Gayle Callis
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