Hi Coni,
We are currently cutting three levels on breast sentinel nodes.  Levels 1 &
3 are stained for H & E. Level 2 is stained for pan-Keratin.  For melanoma
nodes, we cut three slides per block.  One stained S-100, one stained HMB-45
and one H&E.  We are currently reviewing our procedures because we
understand there are other protocols out there which require more levels.  I
would appreciate any and all procedures any of you would like to share and
whether there is a new standard developing.  Thank you.
Best Regards.

Mickie

Michael L. Johnson, HT/HTL(ASCP)
Histology Supervisor
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, Washington 99220
(509) 626-4418
FAX (509) 455-2052
johnsom@shmc.org
-----Original Message-----
From: Coni Forney <conif@MEDCENOA.WPAFB.AF.MIL>
To: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
Date: Friday, July 16, 1999 6:43 AM
Subject: SENTINAL NODE PROTOCOL


>Help - looking for protocol for cutting sentinal nodes - what is everyone
>using as far as levels, etc. We are currently taking several levels at the
>beginning and then every 10 cuts until the block is exhausted. Is there a
>standard protocol or is it site specific? Please advise. Thanks,
>
>Constance M. Forney MT HT(ASCP)
>Technical Supervisor, Histopathology
>USAF Medical Center
>Wright Patterson AFB, OH
>