>From: "Barry, Lilith" <Lilith.Barry@nrc.ca>
>To: "'Histonet'" <histonet@Pathology.swmed.edu>
>Subject: Comments please
>Date: Tue, 20 Jul 1999 15:47:13 -0400
>
>Dear colleagues,
>
>I appreciate very much the numerous replies you have sent to my basic 
>tissue
>processing question. As usual you have supplied the valuable practical
>information which will spare me from learning from my own mistakes. That is
>one of many reasons why the Histonet is so wondeful!  Thank you very much.
>
>There are couple of things that bother me though and would like to share
>with you and be corrected  by you if I am wrong.
>
>One of them is that all the replies came to me personally, that is the
>copies were not sent to the listserver.  So frequently I have seen
>questions posted and have seen very few if any replies to them. I guess 
>mine
>was one of them. True, my question was very basic but still, there might be
>some new histologist that might benefit from the replies.
>
>The second concern is  that among those many helpful replies there was one
>very unprofessional  reply coming from a prominent anatomist. And this one
>was posted, even twice! Most of the subscribers that have read the question
>and have seen only one offensive reply to it. Don't you think that this 
>will
>discourage them from posting questions? If they had seen all the helpful
>replies, the negative one would be easily ignored.
>
>I am eager to read your posted  comments on this.
>
>Lilith
>
>
>Lilith Ohannessian-Barry
>National Research Council
>Institute of Biological Sciences
>CANADA
>Tel;613-993-6460
>Fax;613-941-4475
>e-mail; lilith.barry@nrc.ca <mailto:lilith.barry@nrc.ca>
>Dear Lilith,

Some of the responses on the histonet regarding your queries on processing 
bothered and grieved me.  I have been in this field for over 20 years with 
skills in areas of histology most people never have the  opportunity to 
experience.  I have been fortunate to have great educators that had the 
humility to know they were always teachable. I'll never forget two of the 
comments that were made to me that may be of solace to you.  David Tacha, a 
colleague and mentor, said "The only question not asked is the stupid one 
that may be costly to the patient"; the other was by Lee Luna who told me 
"Histology will always be an emerging science". In other words, histology is 
iThe art and science of cumulative knowledgei.  As long as I try to practice 
these principles in our field, I will continue to be open minded and be of 
service to the patient.

In the areas immunohistochemistry, in-situ hybridization and histology there 
are many unresolved mysteries. Some of these histologic puzzles cannot be 
scientifically substantiated and can only be answered empirically 
(accumulated knowledge through observation).

Inquiry is a pathway to real science, so please keep asking questions!

Vygis Narbutas
Staff Scientist
Biocare Medical



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