BrdU and acridine orange

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From:Deb Boswell Lane <>
Date:Tue, 27 Jul 1999 12:57:46 -0400
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Has anyone heard of a staining procedure for tissue to determine single
stranded vs. double stranded DNA using acridine orange?  We're helping a
lab trouble shoot their BrdU procedure, and this procedure was mentioned.
The single stranded DNA will fluoresce a different color than the double
stranded DNA when stained with acridine orange in this procedure.  Thanks
for any information!

Deb Boswell Lane
The Jackson Laboratory

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