Hi!
Has anyone heard of a staining procedure for tissue to determine single
stranded vs. double stranded DNA using acridine orange?  We're helping a
lab trouble shoot their BrdU procedure, and this procedure was mentioned.
The single stranded DNA will fluoresce a different color than the double
stranded DNA when stained with acridine orange in this procedure.  Thanks
for any information!

Deb Boswell Lane
The Jackson Laboratory