We  have had fairly good success with the kappa/lambda using the following
protocol for paraffin embedded tissue sections:
Enzyme = PROTEASE 1, 12 minutes
option = Option 2, 8 minutes. (additional hydrogen peroxidase treatment 3%)
Primary from Ventana, polyclonal = 32 minutes.

Good Luck, Marg

EMC, Rancho Mirage CA

-----Original Message-----
From: mvaicaitis@lcmh.org [mailto:mvaicaitis@lcmh.org]
Sent: Tuesday, July 27, 1999 7:11 AM
To: histonet@Pathology.swmed.edu
Subject: kappa/lambda


Hi
We have a Ventana ES immunostainer and have not had any success with Kappa
and Lambda.
The Chief says we should try again and I'm hoping someone out there has a
success story to share.
Thanks.

Marija Vaicaitis
Little Co. of Mary Hospital
Evergreen Park, IL