RE: kappa/lambda

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From:"Hagerty, Marjorie A." <>
To:"''" <>,
Date:Tue, 27 Jul 1999 10:50:12 -0700
Content-Type:text/plain; charset="iso-8859-1"

We  have had fairly good success with the kappa/lambda using the following
protocol for paraffin embedded tissue sections:
Enzyme = PROTEASE 1, 12 minutes
option = Option 2, 8 minutes. (additional hydrogen peroxidase treatment 3%)
Primary from Ventana, polyclonal = 32 minutes.

Good Luck, Marg

EMC, Rancho Mirage CA

-----Original Message-----
From: []
Sent: Tuesday, July 27, 1999 7:11 AM
Subject: kappa/lambda

We have a Ventana ES immunostainer and have not had any success with Kappa
and Lambda.
The Chief says we should try again and I'm hoping someone out there has a
success story to share.

Marija Vaicaitis
Little Co. of Mary Hospital
Evergreen Park, IL

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