Re: Inquiring minds and NovaRed
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From: | "vector@vectorlabs.com" <vector@vectorlabs.com> |
To: | "Karen D. Larison" <LARISONK@UONEURO.uoregon.edu>, "Histonet" <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 2 Jul 99 17:10:10 -0700 |
Content-Type: | text/plain; charset="US-ASCII" |
Dear Karen,
Thanks very much for the inquiry. The Vector NovaRED peroxidase substrate
does have similar reaction kinetics to that of DAB, and does not appear
to be water soluble, since the reaction product remains after standing in
tap water. As Gayle has indicated though, and we have confirmed, Vector
NovaRED is not compatible with Immu-Mount. This particular compound,
Immu-Mount, is refered to as an aqueous based mounting media which
contains polyvinyl alcohol, glycerol, amino alcohol, and cationic
quaternary ammonium chloride. This mixture is somewhat more elaborate
than just water or glycerol. As explained previously, Vector Labs have
not evaluated the compatibility of Vector NovaRED with the plethora of
aqueous based mounting medias which are available. The Vector NovaRED was
developed as a crisp, sensitive red peroxidase substrate which can be
dehydrated and permanently mounted in nonaqueous mounting media. Until
now, there has not been a red peroxidase substrate suitable for permanent
mounting. The other red peroxidase substrate, AEC, can be aqueously
mounted, albeit much less sensitive and soluble in alcohols. It should be
indicated that AEC is not stable in all aqueous mountants for long term
storage.
If you were hoping to mount your embryo preparations with an aqueous
compound (glycerol?), please contact Vector Labs and we'll try to
accomodate your inquiry, and trial the Vector NovaRED with your media of
choice. The assumptions by J. A. Kiernan about Vector NovaRED are
incorrect. We are not willing to be involved in a discussion on why some
products are proprietary. Vector Labs is certainly not unique in offering
proprietary products, and it should be made clear that companies such as
Vector manage to stay in business by protecting their intellectual
property. The Vector Technical Service Department is avaiable to assist
you should further questions arise.
Sincerely,
__________
Craig Pow, Ph.D.
Vector Laboratories, Inc.
30 Ingold Road
Burlingame, CA 94010
Tel: (650) 697-3600
Fax: (650) 697-0339
E-mail: craig@vectorlabs.com
Website: http://www.vectorlabs.com
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