Re: CD10

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From:Phyllis Davie <>
To:"Luty, Carolyn" <>, "histonet" <>
Date:Fri, 9 Jul 99 12:55:43 -0700
Content-Type:text/plain; charset="US-ASCII"

We are also using the Novocastra CD10 antibody (clone 56C6, catalog# 
NCL-CD10-270).  We use antigen retrieval in 6mM citrate, pH 6.0, in a 
microwave pressure cooker for 10 minutes at pressure.  The antibody 
dilution is 1:10, and we use Vector ABC Elite detection.  We are 
generally getting good results, but if we have a "weak" day for any 
reason, this antibody is one of the first to suffer.  At 1:10, there 
isn't much room for error.

Hope this helps
Phyllis Davie
PhenoPath Laboratories

>Sorry if this is a repeat question but we are having problems with CD10
>on paraffin sections. Does anyone have any suggestions? We used antigen
>retrieval with citrate buffer but it still did not work. It is a
>Novacastro antibody.
>Montreal General Hospital

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