RE: [Histonet] trichrome problems

From:"Sherwood, Margaret "

We routinely leave our slides in a 60 degree oven overnight before staining.

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 

-----Original Message-----
[]On Behalf Of Derek
Sent: Thursday, August 02, 2007 10:28 AM
Subject: [Histonet] trichrome problems

Hi Everyone,
I am having trouble with the tissue staying on the slide when doing a 
trichrome stain. I currently use the Richard Allen super up-rite slides. 
I found that the tissue came off the slide after I put it in the heated 
bouin's solution for an hour. I tried to leave it in room temp bouin's 
overnight instead of using it heated and I found that some tissue has 
stayed on but some has still fallen off. I have tried both leaving 
slides out to dry overnight as well as cutting and staining on the same 
day. Does anyone have any ideas of how I can solve this problem?


Derek Papalegis HT (ASCP)
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354

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