Derek,
Our tissue are collected onto charged slides (Fisherbrand SuperFrost) and
allowed to adhere overnight on the slide warmer at ~37C.
I had the very same problem when I was trichrome staining. I found that 1hr
in the heated Bouins fixative caused the tissue to come loose from the
slide. I had much better luck with the overnight fix at room temperature and I
haven't had any significant problems since I started using the O/N post-fix.
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
************************************** Get a sneak peek of the all-new AOL at
http://discover.aol.com/memed/aolcom30tour
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|