I've been doing histology for only about a year and am in the middle of a study on reproductive biology of goatfish, Mullidae, and most of my stuff has turned out well, with only minor problems. But, my question is this...does anyone know a reliable source to which I can send preserved ovaries to be embedded, sectioned, made into slides and stained with H & E?? I've been doing this myself but it would cut a lot of time out of my project if I could send them out, and unfortunately our university's histological prowess is lacking. A fellow grad student of mine said she'd sent them to Louisiana State I believe, but I cannot get a hold of her. Any suggestions would be welcomed!
University of Hawaii, Manoa
2538 The Mall, 152 Edmondson Hall
Honolulu, HI 96822
----- Original Message -----
From: Rene J Buesa
Date: Wednesday, July 25, 2007 6:28 am
Subject: Re: [Histonet] hemoglobin
To: Derek Papalegis , email@example.com
> There is a method (Ralph, 1941) that uses 1% benzidine. I would
> NOT use since benzidine is highly toxic.
> Goulliart (1941) uses acetic acid with KI to later examine under
> polarized light (looking for the so called Teichmann crystals).
> Dunn (1946) stains frozen sections with a rippen solution of
> cyanol in acidified water, counterstained with an acid safranin
> solution. O'Brien (1961) describes a method using the hemoglobin
> catalase reaction with peroxide and o-dianisidine.
> All these methods could make "havoc" into your well structured
> and "safe" routine. Try to convince your investigator that Prussian
> blue is enough (that is what I would do).
> René J.
> Derek Papalegis wrote:
> Hi All,
> An investigator has asked me to stain some sections for hemoglobin.
> have already provided him with an iron stain and he wants to go
> with it. Can anyone recommend a stain specifically for hemoglobin?
> have found some for hemosiderin but I am unsure as to if they will
> sufficient. If someone could let me know what stain they use and
> the procedure is I would greatly appreciate it.
> Derek Papalegis HT (ASCP)
> Division of Laboratory Animal Medicine
> Tufts University
> 136 Harrison Avenue
> Boston, MA 02111
> phone: 617 636-2971
> fax: 617 636-8354
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