Please unsubscibe me to histonet. Thaks jane Flinn
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Biopsychology Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA 22030
Phone: 703-993-4107
Fax: 703-993-1359
----- Original Message -----
From: Heather A Leba
Date: Wednesday, July 25, 2007 9:59 pm
Subject: [Histonet] histology samples
> Hello all,
>
> I've been doing histology for only about a year and am in the
> middle of a study on reproductive biology of goatfish, Mullidae,
> and most of my stuff has turned out well, with only minor
> problems. But, my question is this...does anyone know a reliable
> source to which I can send preserved ovaries to be embedded,
> sectioned, made into slides and stained with H & E?? I've been
> doing this myself but it would cut a lot of time out of my project
> if I could send them out, and unfortunately our university's
> histological prowess is lacking. A fellow grad student of mine
> said she'd sent them to Louisiana State I believe, but I cannot
> get a hold of her. Any suggestions would be welcomed!
>
> Thanks!
>
> Heather Leba
>
> Graduate Student
> University of Hawaii, Manoa
> 2538 The Mall, 152 Edmondson Hall
> Honolulu, HI 96822
>
>
> ----- Original Message -----
> From: Rene J Buesa
> Date: Wednesday, July 25, 2007 6:28 am
> Subject: Re: [Histonet] hemoglobin
> To: Derek Papalegis ,
> histonet@lists.utsouthwestern.edu
> > Derek:
> > There is a method (Ralph, 1941) that uses 1% benzidine. I would
> > NOT use since benzidine is highly toxic.
> > Goulliart (1941) uses acetic acid with KI to later examine
> under
> > polarized light (looking for the so called Teichmann crystals).
> > Dunn (1946) stains frozen sections with a rippen solution of
> > cyanol in acidified water, counterstained with an acid safranin
> > solution. O'Brien (1961) describes a method using the
> hemoglobin
> > catalase reaction with peroxide and o-dianisidine.
> > All these methods could make "havoc" into your well structured
> > and "safe" routine. Try to convince your investigator that
> Prussian
> > blue is enough (that is what I would do).
> > Ren� J.
> >
> > Derek Papalegis wrote:
> > Hi All,
> > An investigator has asked me to stain some sections for
> hemoglobin.
> > I
> > have already provided him with an iron stain and he wants to go
> > further
> > with it. Can anyone recommend a stain specifically for
> hemoglobin?
> > I
> > have found some for hemosiderin but I am unsure as to if they
> will
> > be
> > sufficient. If someone could let me know what stain they use and
> > what
> > the procedure is I would greatly appreciate it.
> >
> > Thanks!
> > -Derek
> >
> > --
> > Derek Papalegis HT (ASCP)
> > Histotechnician
> > Division of Laboratory Animal Medicine
> > Tufts University
> > 136 Harrison Avenue
> > Boston, MA 02111
> > phone: 617 636-2971
> > fax: 617 636-8354
> >
> >
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> >
> >
> >
> >
> >
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