Re: [Histonet] histology samples

From:Jane M Flinn



Please unsubscibe me to histonet. Thaks jane Flinn

"Life is short - make haste to be kind"

Dr. Jane Flinn
Director, Biopsychology Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA  22030
Phone: 703-993-4107
Fax: 703-993-1359


----- Original Message -----
From: Heather A Leba 
Date: Wednesday, July 25, 2007 9:59 pm
Subject: [Histonet] histology samples

> Hello all, 
> 
> I've been doing histology for only about a year and am in the 
> middle of a study on reproductive biology of goatfish, Mullidae, 
> and most of my stuff has turned out well, with only minor 
> problems. But, my question is this...does anyone know a reliable 
> source to which I can send preserved ovaries to be embedded, 
> sectioned, made into slides and stained with H & E?? I've been 
> doing this myself but it would cut a lot of time out of my project 
> if I could send them out, and unfortunately our university's 
> histological prowess is lacking. A fellow grad student of mine 
> said she'd sent them to Louisiana State I believe, but I cannot 
> get a hold of her. Any suggestions would be welcomed! 
> 
> Thanks!
> 
> Heather Leba
> 
> Graduate Student
> University of Hawaii, Manoa
> 2538 The Mall, 152 Edmondson Hall
> Honolulu, HI 96822
> 
> 
> ----- Original Message -----
> From: Rene J Buesa 
> Date: Wednesday, July 25, 2007 6:28 am
> Subject: Re: [Histonet] hemoglobin
> To: Derek Papalegis , 
> histonet@lists.utsouthwestern.edu
> > Derek:
> >  There is a method (Ralph, 1941) that uses 1% benzidine. I would 
> > NOT use since benzidine is highly toxic.
> >  Goulliart (1941) uses acetic acid with KI to later examine 
> under 
> > polarized light (looking for the so called Teichmann crystals).
> >  Dunn (1946) stains frozen sections with a rippen solution of 
> > cyanol in acidified water, counterstained with an acid safranin 
> > solution.  O'Brien (1961) describes a method using the 
> hemoglobin 
> > catalase reaction with peroxide and o-dianisidine.
> >  All these methods could make "havoc" into your well structured 
> > and "safe" routine. Try to convince your investigator that 
> Prussian 
> > blue is enough (that is what I would do).
> >  Ren´┐Ż J.
> > 
> > Derek Papalegis  wrote:
> >  Hi All,
> > An investigator has asked me to stain some sections for 
> hemoglobin. 
> > I 
> > have already provided him with an iron stain and he wants to go 
> > further 
> > with it. Can anyone recommend a stain specifically for 
> hemoglobin? 
> > I 
> > have found some for hemosiderin but I am unsure as to if they 
> will 
> > be 
> > sufficient. If someone could let me know what stain they use and 
> > what 
> > the procedure is I would greatly appreciate it.
> > 
> > Thanks!
> > -Derek
> > 
> > -- 
> > Derek Papalegis HT (ASCP)
> > Histotechnician
> > Division of Laboratory Animal Medicine
> > Tufts University 
> > 136 Harrison Avenue
> > Boston, MA 02111
> > phone: 617 636-2971
> > fax: 617 636-8354
> > 
> > 
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> > 
> > 
> > 
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