We have been using a goat pAb to Oct-3/4 (C-20) from Santa Cruz with good results.  We use the antibody at a dilution of 1:100 (for 30 minutes) following heat retrieval.  The only issue is that the antibody is labeled "RUO".

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Dana Settembre"  07/16/07 7:14 AM >>>
Hello,
I am looking to find if any one is using or has used Oct-4
on formalin fixed paraffin embedded human tissue.
None of the vendors I usually use seem to have it.
What vendor?  Dilution would be great too.
Thank you for any help.

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Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


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