Hi Dana,

We are using Santa Cruz's OCT 3/4 at 1:100 with Ventana's standard CC1
HIER solution on their instruments or 10" Biocare's BORG HIER solution
in a pressure cooker, which is as harsh as we get for off-line
retrieval.  If you have any more questions let us know.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Monday, July 16, 2007 6:15 AM
To: springrosycloud@126.com; Ronald Houston;
histonet@lists.utsouthwestern.edu
Cc: Catherine Susan Delia; Meera Hameed
Subject: [Histonet] Oct-4, looking for it


Hello,
I am looking to find if any one is using or has used Oct-4
on formalin fixed paraffin embedded human tissue.
None of the vendors I usually use seem to have it.
What vendor?  Dilution would be great too.
Thank you for any help.

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Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


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