Re: [Histonet] Request for a copy of pages on GFP visualization in FFPE tissue
I sent you the copy.
Hope this help!
--- Gayle Callis wrote:
> Dear all,
> If anyone has this manual, could you send a copy of
> Page 696 on protocol
> for fixation and paraffin embedding for GFP
> Manipulating the Mouse Embryo: A Laboratory
> Manual, 3rd ed.
> Andras Nagy, Marina Gertsenstein, Kristina
> Vintersten, and Richard
> Behringer. 2003.
> Cold Spring Harbor Laboratory Press.
> ISBN 0-87969-574-9
> page 696 for protocol on fixation and paraffin
> embedding for GFP visualization.
> We do not have the manual in our laboratory and are
> doing some outside
> processing for other people.
> Also, I would entertain any comments on success or
> lack of success on this
> subject. I has been discussed before, but methods
> improve over the years
> on maintaining eGFP fluorescence after FFPE. I
> would appreciate detailed
> processing schedules, in particular, use of
> vacuum/pressures, times in
> solvents, and paraffin temperatures. You can send
> it privately if you wish.
> Thanks in advance
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> Histonet mailing list
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