Re: [Histonet] Amino Silane coated slides

From:Amy Porter

Sharon this is what we use in our lab:
2% 3-Aminopropyltriethoxysilane (Sigma)  Cat #:  A3648
Comercially purchased Silane   15.0 mls
Acetone (reagent grade)         750.0 mls
Prepare fresh each use.
Submerge slides in solution for 2 minutes, Rinse well in two changes of distilled water,
tap excess water off of racks and stand slides with frosted end down at an angle and allow to air dry.  Ready to use once dry.
reference:  Weetal, H.H. Biochem.Biphys.  ACTA, 212:1 (1970); Histochemical Journal 1986:  18, 271-276.

"Sharon.Davis-Devine"  wrote:
I am looking for anyone who would be willing to share their protocol for
coating of Amino Silane slides. Thanks in advance.

Sharon Davis-Devine, CT (ASCP)

Technical Specialist of Cytology

Carle Clinic

602 West University Ave.

Urbana, Illinois 61801

Phone: 217-383-3402

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Amy S.Porter, HT(ASCP) 
Michigan State University 
Department of Physiology 
Division of Human Pathology 
College of Human Medicine

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