[Histonet] staining compatibility
Hi everyone,
I am a new subscriber, am glad there are so many experts around can offer
help. I am a medical student doing summer research. We use simplified H&E
stain before LCM (Laser capture microdissection) with subsequent proteomic
analysis.
I stumbled upon this journal: Electrophoresis 2003, 24, 296-302, which
claims that conventional histological staining methods are not compatible
with 2-dimentional gel electrophoresis. While another paper: American
Journal of Pathology 2002, 160:815-822 claims that those histochemical
stains is compatible with such protein analysis, variations for the relative
intensity and reproducibility of proteins is observed, though.
These two opinions seems to me a little contradictory and I am currently
working on it, trying to establish a master gel which other Laser captured
gel can compare to.
Any suggestion is highly welcome, thanks in advance.
----------------------------------------------------------------
Yi-hsin Wang
Department of Medicine
Kaohsiung Medical University
100 Shi-Chuan 1st Road, San Ming District, Kaohsiung City, Taiwan
yhwang0731@hotmail.com
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